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Registros recuperados: 27
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Effects of diets with increasing levels of citrus pulp on the blood parameters linked to energy metabolism in horses Ciência e Agrotecnologia
Menezes,Madalena Lima; Brandi,Roberta Ariboni; Bueno,Ives Cláudio da Silva; Balieiro,Júlio Cesar de Carvalho; Moreira,Camilla Garcia; Nascimento,Olivia Carmen de Araújo.
Currently, the use of alternative energy ingredients for horses has increased because these foods contain "superfibers", making safer diet for these animals. To study the influence of diets containing increasing levels of citrus pulp on albumin, triglycerides, cholesterol, glucose, insulin and short chain fatty acid (SCFA) concentrations in the blood, five animals were used, with an average age of 3.5 years and a live weight of 460.66±76.86 kg, they were fed twice a day, at 7:00 am and 4:00 pm. The diets were formulated to meet the requirements of the animals at maintenance. 60% of the energy was obtained from forage, and 40% from concentrate, containing the following inclusion levels of citrus pulp (0, 7, 14, 21 and 28%). The area under the curve (AUC)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Soluble fiber; Nutrition; Pectin; Plasma.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542014000600008
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Glyphosate-based herbicide affects biochemical parameters in Rhamdia quelen (Quoy & Gaimard, 1824 and) Leporinus obtusidens (Valenciennes, 1837) Neotropical Ichthyology
Loro,Vania Lucia; Glusczak,Lissandra; Moraes,Bibiana S.; Leal,Claudio A. M.; Menezes,Charlene; Murussi,Camila R.; Leitemperger,Jossiele; Schetinger,Maria Rosa C.; Morsch,Vera Maria.
Rhamdia quelen (silver catfish) and Leporinus obtusidens (piava) were exposed to a commercial formulation Roundup(r), a glyphosate-based herbicide at concentrations of 0.2 or 0.4 mg/L for 96 h. The effects of the herbicide were analyzed on the alanine aminotransferase (ALT) and aspartate aminotransferase (AST) activities and glucose in plasma, glucose and protein in the mucus layer, nucleotide hydrolysis in the brain, and protein carbonyl in the liver. The parameters were chosen, owing to a lack of information concerning integrated analysis, considering oxidative damage parameters, liver damage, and effects on the mucus layer composition and triphosphate diphosphohydrolase (NTPDase) activities. Plasmatic glucose levels were reduced in both species, whereas...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish; Mucus; Nucleotidases; Plasma; Protein carbonyl.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252015000100229
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Specific alterations in plasma proteins during depressed, manic, and euthymic states of bipolar disorder BJMBR
Song,Y.R.; Wu,B.; Yang,Y.T.; Chen,J.; Zhang,L.J.; Zhang,Z.W.; Shi,H.Y.; Huang,C.L.; Pan,J.X.; Xie,P..
Bipolar disorder (BD) is a common psychiatric mood disorder affecting more than 1-2% of the general population of different European countries. Unfortunately, there is no objective laboratory-based test to aid BD diagnosis or monitor its progression, and little is known about the molecular basis of BD. Here, we performed a comparative proteomic study to identify differentially expressed plasma proteins in various BD mood states (depressed BD, manic BD, and euthymic BD) relative to healthy controls. A total of 10 euthymic BD, 20 depressed BD, 15 manic BD, and 20 demographically matched healthy control subjects were recruited. Seven high-abundance proteins were immunodepleted in plasma samples from the 4 experimental groups, which were then subjected to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bipolar disorder; Plasma; Proteomic; Carbonic anhydrase 1; Apolipoprotein.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015001100973
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Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination BJPS
Razzaq,Syed Naeem; Ashfaq,Muhammad; Khan,Islam Ullah; Mariam,Irfana; Razzaq,Syed Saleem; Mustafa,Ghulam; Zubair,Muhammad.
Abstract In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (42:58 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gatifloxacin; Dexamethasone; High performance liquid chromatography; Validation; Plasma; Degradation products..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000100607
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Development of a new HPLC method for in vitro and in vivo studies of haloperidol in solid lipid nanoparticles BJPS
Yasir,Mohd; Sara,Udai Vir Singh; Som,Iti.
ABSTRACT A simple and sensitive HPLC method was developed and validated for the quantification of haloperidol in solid lipid nanoparticles (SLNs). The developed method was used for detection of shelf life of haloperidol in SLNs. Calibration curve of haloperidol was also constructed in rat plasma using loratidine as internal standard. In vivo studies were performed on rats and concentration of haloperidol in brain and blood was measured for the determination of various pharmacokinetic and hence brain targeting parameters. Chromatogram separation was achieved using C18 column as stationary phase. The mobile phase consisted of 100 mM/L potassium dihydrogen phosphate-acetonitrile-TEA (10:90:0.1, v/v/v) and the pH was adjusted with o-phosphoric acid to 3.5....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Haloperidol/quantification; Haloperidol/pharmacokinetic; Haloperidol/in vitro study; Haloperidol/in vivo study; High performance liquid chromatography/solid lipid nanoparticles; Plasma; Validation; Brain targeting..
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502017000200623
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Determination of Gemifloxacin in human plasma by high performance liquid chromatography using Ultra Violet detector and its application to a bioequivalence study BJPS
Mousavi,Syed Husain Hashemi.
A liquid chromatography method was developed and validated for the determination of gemifloxacin in human plasma using chloramphenicol as internal standard to achieve lower quantification limit. Acetonitrile was used to precipitated and extracted analyte and internal standard from plasma by Protein Precipitation. Analysis was performed isocratically on C18 column using 25% acetonitrile and 75% 0.02 M phosphate buffer as mobile phase. The method was demonstrated to be linear from 0.003 µg/mL to 5 µg/mL with the lower limit of quantitation of 0.003 µg/mL. The method was successfully applied for the bioequivalence study of gemifloxacin after a single oral administration of 320 mg gemifloxacin mesylate tablets to 12 healthy volunteers.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Gemifloxacin/determination; Bioequivalence; Plasma; HPLC.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502018000400610
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Plasma miR-1273g-3p acts as a potential biomarker for early Breast Ductal Cancer diagnosis Anais da ABC (AABC)
Guo,Huan; Zeng,Xiangting; Li,Haining; Guo,Yanxiang; Wang,Tao; Guo,Hongyun; Zhu,Gongjian; Wang,Lan; Zhou,Haihong; Liu,Kedan; Chen,Xiaoyi; Wang,Haitao; Zhao,Xinke; Su,Haixiang; Li,Yingdong.
Abstract Circulating miRNAs presenting in plasma in a stable manner have been demonstrated their potential role as a promising biomarkers in many human diseases, such as Alzheimer’s disease, melanoma and ovarian carcinoma. However, few circulating miRNAs could be used for breast ductal cancer diagnosis. Here, we identified miR-1273g-3p as a biomarker for detecting breast ductal cancer. We detected miR-1273g-3p levels in the plasma of 39 sporadic breast ductal cancer patients and 40 healthy donors by Stem-loop Quantitative Real-time PCR (qRT-PCR). The results showed the plasma miR-1273g-3p level were significantly up-regulated in breast ductal cancer patients compared with healthy donors (p=0.0139). Receiver operating characteristic (ROC) curve also...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Breast ductal carcinoma; Plasma; MiR-1273g-3p; Circulating miRNA; Biomarker.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100801
Registros recuperados: 27
Primeira ... 12 ... Última
 

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